| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Tri-Cities United Soccer Club Tri-Cities United 06 Boys Premier |
0106-011B7U-2246 | J Burns |
| A2 | Lobos Rush (Collierville Soccer Association) 2006 Lobos Rush E64 |
0603-011B7U-0012 | Clint Browne |
| A3 | Nashville United Soccer Academy - 01 NUSA B06 Maroon |
0534-011B7U-0011 | Ethan Keogh |
| A4 | Tennessee United Soccer Club TUSC 2006 Boys NPL |
0424-011B7U-0010 | Nate Sego |
| A5 | Tennessee Soccer Club TSC Murfreesboro 2006 Boys Showcase |
0522-011B7U-0135 | Jason Finley |
| A6 | Clarksville Soccer Club Clarksville SC - B2006 Maroon |
0422-011B7U-0014 | Scott Dollar |
| A7 | Midsouth Futbol Midsouth Futbol Boys 2006 Premier |
0654-011B7U-0001 | Carl Schmitt |
| A8 | Jackson Soccer Club JSC 2006 Boys |
0609-012B7U-0006 | Malang Jarju |