| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Germantown Legends Germantown Legends 05 Black NL |
0606-011B8U-0010 | Phil McDonnell |
| A2 | Tennessee Soccer Club TSC Murfreesboro 2005 Boys Showcase |
0522-011B8U-0139 | Enrique Suarez |
| A3 | Tennessee United Soccer Club TUSC 2005 Boys NPL |
0424-011B8U-0011 | Jamie Smith |
| A4 | Signal Mountain Soccer League - 01 Signal Mountain 2005 Boys |
0314-013B8U-0002 | Robert Nix |
| A5 | Nashville United Soccer Academy - 01 NUSA B05 Maroon |
0534-011B8U-0012 | Daniel O'Hare |
| A6 | Pateadores Soccer Club - Nashville Pateadores 05 Blue |
0500-01D1B8U-0066 | Camilo Speranza |
| A7 | Kononia Soccer Club - 01 Kononia FC FIRE |
0611-011B8U-1981 | Sean Cogan |
| A8 | Midsouth Futbol Midsouth Futbol Boys 2005 Premier |
0654-011B8U-0039 | William Pryor |