| Group | Club/Team Name | Team ID | Head Coach |
| A1 | Germantown Legends Germantown Legends 04 Black NL |
0606-011B9U-0011 | Carey Talley |
| A2 | Nashville United Soccer Academy - 01 NUSA B04 Maroon |
0534-011B9U-0013 | John Sintic |
| A3 | Play Where You Stay - 01 PWYS '04 Boys |
0653-011B9U-0003 | Maximiliano Galizzi |
| A4 | ALBION SC Nashville (Formerly Southern Premier Soccer) Albion SC Nashville B04 Academy |
0540-02D2B9U-0023 | Brent Goulet |
| A5 | Lobos Rush (Collierville Soccer Association) 2004/05 Lobos Rush E64 |
0603-011B9U-0054 | Carlos Rivera |
| A6 | Tri-Cities United Soccer Club Tri-Cities United 04/05 Boys Premier |
0106-011B9U-2237 | Nicolas Boegemann |
| A7 | Stones River Futbol Club - 01 Stones River FC 2004 Black |
0521-011B9U-2080 | Jun Taulbee |
| A8 | Jackson Soccer Club JSC 2004 Boys |
0609-012B9U-0005 | Musa Manneh |